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Gene Expr Patterns. 2003 Mar;3(1):105-8.

Expression of isotocin-neurophysin mRNA in developing zebrafish.

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Department of Neurobiology and Pharmacology, Northeastern Ohio Universities College of Medicine, Rootstown, OH 44272, USA.


Neurohypophysial peptides are important regulators of homeostasis, reproduction and behavior. We have sequenced a zebrafish cDNA representing isotocin-neurophysin (IT-NP) mRNA. The developmental expression pattern of zebrafish IT-NP mRNA was determined by whole-mount in situ hybridization histochemistry. At 32 h post fertilization (hpf) no IT-NP mRNA is detected. However, by 36 hpf, staining for IT-NP mRNA is detected in a tight bilateral cluster of cells located in the anterior hypothalamus. The IT-NP mRNA expression pattern remains remarkably stable throughout further development at least until 120 hpf.

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