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Biopolymers. 2003 Mar;68(3):286-99.

Computer simulation studies of the fidelity of DNA polymerases.

Author information

1
Department of Chemistry, University of Southern California, Los Angeles, CA 90089-1062, USA.

Abstract

Computer simulations can provide in principle quantitative correlation between the structures of DNA polymerases and the replication fidelity. This paper describes our progress in this direction. Using several theoretical approaches, including the free energy perturbation (FEP), linear response approximation (LRA), and the empirical valence bond (EVB) methods, we examined the stability of several mismatched base pairs in DNA duplex in aqueous solution, the contribution of binding energy to the fidelity of DNA polymerases beta and T7, and the mechanism and energetics of the polymerization reaction catalyzed by T7 DNA polymerase.

PMID:
12601790
DOI:
10.1002/bip.10244
[Indexed for MEDLINE]

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