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Cell. 2003 Feb 21;112(4):453-65.

Cellular motility driven by assembly and disassembly of actin filaments.

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1
Department of Cellular, Molecular, and Developmental Biology, Yale University, New Haven, CT 06520, USA. thomas.pollard@yale.edu

Erratum in

  • Cell. 2003 May 16;113(4):549.

Abstract

Motile cells extend a leading edge by assembling a branched network of actin filaments that produces physical force as the polymers grow beneath the plasma membrane. A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematical models of the constituent reactions predict the rate of motion. Signaling pathways converging on WASp/Scar proteins regulate the activity of Arp2/3 complex, which mediates the initiation of new filaments as branches on preexisting filaments. After a brief spurt of growth, capping protein terminates the elongation of the filaments. After filaments have aged by hydrolysis of their bound ATP and dissociation of the gamma phosphate, ADF/cofilin proteins promote debranching and depolymerization. Profilin catalyzes the exchange of ADP for ATP, refilling the pool of ATP-actin monomers bound to profilin, ready for elongation.

PMID:
12600310
[Indexed for MEDLINE]
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