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Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):433-7. Epub 2003 Feb 21.

Crystal structure of rGd(CGCGCG): a Z-DNA hexamer duplex with a 5'-(rG) overhang.

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1
Departments of Chemistry and Biochemistry, 200 Johnston Laboratory, 176 West 19th Avenue, Columbus, Ohio 43210-1002, USA.

Abstract

The crystal structure of the heptamer rGd(CGCGCG) has been determined at 1.54 A resolution with R(work) and R(free) of 0.191 and 0.235, respectively. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 17.96, b = 31.47, c = 44.73 A and two independent strands in the asymmetric unit. The chimera forms a Z-DNA hexamer duplex d(CGCGCG)(2), with the 5'-overhang rG invisible in the density. The replacement of rG in the 5' terminus of d(GCGCGCG) changes the reverse Hoogsteen G.G base pairing of the 5'-overhang dG (Pan et al., 1997). However, the replacement does not change the helix from Z-form to A-form or the water-structure motifs in the Z-DNA double-helical structure.

PMID:
12595699
[Indexed for MEDLINE]
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