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J Bacteriol. 2003 Mar;185(5):1719-25.

DNA microarray analysis of redox-responsive genes in the genome of the cyanobacterium Synechocystis sp. strain PCC 6803.

Author information

1
Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, Saitama-shi, Saitama 338-8570, Japan. hihara@molbiol.saitama-u.ac.jp

Abstract

Whole-genome DNA microarrays were used to evaluate the effect of the redox state of the photosynthetic electron transport chain on gene expression in Synechocystis sp. strain PCC 6803. Two specific inhibitors of electron transport, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), were added to the cultures, and changes in accumulation of transcripts were examined. About 140 genes were highlighted as reproducibly affected by the change in the redox state of the photosynthetic electron transport chain. It was shown that some stress-responsive genes but not photosynthetic genes were under the control of the redox state of the plastoquinone pool in Synechocystis sp. strain PCC 6803.

PMID:
12591891
PMCID:
PMC148065
[Indexed for MEDLINE]
Free PMC Article

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