Extraction of Chlamydia pneumoniae DNA from vascular tissue for use in PCR: an evaluation of four procedures

H F Berg; B Maraha; A M C Bergmans; A van der Zee; J A J W Kluytmans; M F Peeters

doi:10.1046/j.1469-0691.2003.00470.x

Extraction of Chlamydia pneumoniae DNA from vascular tissue for use in PCR: an evaluation of four procedures

Clin Microbiol Infect. 2003 Feb;9(2):135-9. doi: 10.1046/j.1469-0691.2003.00470.x.

Authors

H F Berg¹, B Maraha, A M C Bergmans, A van der Zee, J A J W Kluytmans, M F Peeters

Affiliation

¹ Department of Medical Microbiology, St Elisabeth Hospital, PO Box 747, 5000 AS Tilburg, the Netherlands.

PMID: 12588334
DOI: 10.1046/j.1469-0691.2003.00470.x

Abstract

The objective of this study was to compare four procedures for Chlamydia pneumoniae DNA extraction from vascular tissue. The NucliSens Kit, the QIAamp tissue DNA MiniKit, buffer-saturated phenol and the Geneclean II Kit were evaluated, based on the yield of recovered DNA, using PCR to detect C. pneumoniae in vascular tissue. The QIAamp tissue procedure had the highest detection level (0.004 inclusion-forming units/sample). All methods, except NucliSens (70 min), had a short handling time (30-40 min). Costs varied from 0.5 to 3.2 Euro.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Chlamydia Infections / pathology*
Chlamydophila pneumoniae / genetics*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Bacterial / isolation & purification*
Humans
Muscle, Smooth, Vascular / microbiology*
Nucleic Acid Hybridization
Polymerase Chain Reaction / economics
Polymerase Chain Reaction / methods*

Substances

DNA, Bacterial