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FEMS Microbiol Lett. 2003 Jan 28;218(2):223-30.

Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602.

Author information

1
Kosan Biosciences, 3832 Bay Center Place, 94545, Hayward, CA, USA. rascher@kosan.com

Abstract

We illustrate the use of a PCR-based method by which the genomic DNA of a microorganism can be rapidly queried for the presence of type I modular polyketide synthase genes to clone and characterize, by sequence analysis and gene disruption, a major portion of the geldanamycin production gene cluster from Streptomyces hygroscopicus var. geldanus NRRL 3602.

PMID:
12586396
DOI:
10.1016/S0378-1097(02)01148-5
[Indexed for MEDLINE]
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