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FEMS Microbiol Lett. 2003 Jan 21;218(1):65-70.

Mutations in sit B and sit D genes affect manganese-growth requirements in Sinorhizobium meliloti.

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Departamento de Bioquímica, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Av Italia 3318, Montevideo 11600, Uruguay.


Two transposon-induced mutants of Sinorhizobium meliloti 242 were isolated based on their inability to grow on rich medium supplemented with the metal chelator ethylenediamine di-o-hydroxyphenylacetic acid (EDDHA) and either heme-compounds or siderophores as iron sources. Tagged loci of these mutants were identified as sit B and sit D genes. These genes encode components of an ABC (ATP-binding cassette) metal-type permease in several Gram-negative bacteria. In this work, the phenotypes of these two mutants were compared with those of two siderophore-mediated iron transport mutants. The results strongly implicate a role of the sit genes in manganese acquisition when this metal is limiting in S. meliloti.

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