Format

Send to

Choose Destination
FEMS Microbiol Lett. 2003 Jan 21;218(1):65-70.

Mutations in sit B and sit D genes affect manganese-growth requirements in Sinorhizobium meliloti.

Author information

1
Departamento de Bioquímica, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Av Italia 3318, Montevideo 11600, Uruguay.

Abstract

Two transposon-induced mutants of Sinorhizobium meliloti 242 were isolated based on their inability to grow on rich medium supplemented with the metal chelator ethylenediamine di-o-hydroxyphenylacetic acid (EDDHA) and either heme-compounds or siderophores as iron sources. Tagged loci of these mutants were identified as sit B and sit D genes. These genes encode components of an ABC (ATP-binding cassette) metal-type permease in several Gram-negative bacteria. In this work, the phenotypes of these two mutants were compared with those of two siderophore-mediated iron transport mutants. The results strongly implicate a role of the sit genes in manganese acquisition when this metal is limiting in S. meliloti.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for Wiley
Loading ...
Support Center