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Nucleic Acids Res. 2003 Feb 15;31(4):e12.

Rapid generation of inducible mouse mutants.

Author information

1
ARTEMIS Pharmaceuticals GmbH, Neurather Ring 1, 51063 Cologne, Germany.

Abstract

We have generated an optimized inducible recombination system for conditional gene targeting based on a Cre recombinase-steroid receptor fusion. This configuration allows efficient Cre-mediated recombination in most organs of the mouse upon induction, without detectable background activity. An ES cell line, was established that carries the inducible recombinase and a loxP-flanked lacZ reporter gene. Out of this line, completely ES cell-derived mice were efficiently produced through tetraploid blastocyst complementation, without the requirement of mouse breeding. Our findings provide a new concept allowing the generation of inducible mouse mutants within 6 months, as compared to 14 months using the current protocol.

PMID:
12582257
PMCID:
PMC150244
DOI:
10.1093/nar/gng012
[Indexed for MEDLINE]
Free PMC Article

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