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Obes Res. 2003 Feb;11(2):176-82.

Reduced PDK4 expression associates with increased insulin sensitivity in postobese patients.

Author information

1
Department of Internal Medicine, Surgery, and Consiglio Nazionale della Ricerca (CNR) Center Catholic University of the Sacred Heart, Rome, Italy. guisi_rosa3@hotmail.com

Abstract

OBJECTIVE:

The aim of this study was to verify whether changes in PDK4 mRNA expression in skeletal muscle in formerly obese subjects who underwent malabsorptive bariatric surgery [bilio-pancreatic diversion (BPD)] might be related to insulin sensitivity improvement, and if these possible modifications might correlate with a reduction of the intramyocytic lipid level.

RESEARCH METHODS AND PROCEDURES:

Six obese women (body mass index 46.6 +/- 8.2 kg/m(2)) were enrolled in the study. Body composition, euglycemic-hyperinsulinemic clamp and muscle biopsies for skeletal muscle lipid analysis, and semiquantitative reverse transcriptase-polymerase chain reaction were performed before and 3 years after BPD.

RESULTS:

The average weight loss observed after surgery was approximately 42%. Increased glucose uptake was accompanied by a significant decrease of PDK4 mRNA (R(2) = 0.71, p < 0.001). The amounts of intramyocytic triglycerides correlate directly with PDK4 mRNA (R(2) = 0.87, p = 0.005) and inversely with glucose uptake values (R(2) = 0.75, p < 0.001).

DISCUSSION:

Our results support the concept that a reduced tissue availability of fatty acids consequent to a massive lipid malabsorption influences glucose metabolism acting through the regulation of PDH complex. In fact, as shown in animals, a higher level of FFA availability is likely to induce overexpression of PDK4 also in humans.

PMID:
12582211
DOI:
10.1038/oby.2003.28
[Indexed for MEDLINE]
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