[Determination of annonaceous acetogenins in annonaceae plants by HPLC]

Yao Xue Xue Bao. 2001 Sep;36(9):683-5.
[Article in Chinese]

Abstract

Aim: To develop a method for analysis of antitumor annonaceous acetogenins in Annonaceae plants by HPLC.

Methods: Squamostatin-B (1), squamocin (2) and annonin-VI (3) were used as standard substances. Chromatography column was a Rp-18; the mobile phase was methanol-water (90:10); the flow rate was 1.0 mL.min-1 and the detecting wavelength was 220 nm.

Results: A linear range was obtained from 2.3 to 13.8 micrograms with a good correlation. The recoveries of (1), (2) and (3) were 100.3%, 100.3% and 100.0%, respectively.

Conclusion: This method was developed for the analysis of acetogenins by HPLC for the first time. The method is rapid, accurate and suitable for the analysis of the antitumor acetogenins in Annonaceae plants.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 4-Butyrolactone / analogs & derivatives
  • 4-Butyrolactone / analysis*
  • 4-Butyrolactone / chemistry
  • Annona / chemistry*
  • Annonaceae / chemistry*
  • Chromatography, High Pressure Liquid
  • Fatty Alcohols / analysis*
  • Fatty Alcohols / chemistry
  • Furans / analysis*
  • Furans / chemistry
  • Lactones / analysis*
  • Lactones / chemistry
  • Molecular Structure
  • Plants, Medicinal / chemistry
  • Seeds / chemistry

Substances

  • Fatty Alcohols
  • Furans
  • Lactones
  • squamostatin B
  • squamocin
  • annonin VI
  • 4-Butyrolactone