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Diagn Microbiol Infect Dis. 2003 Jan;45(1):23-7.

Detection of group B streptococcal bacteremia in simulated intrapartum antimicrobial prophylaxis.

Author information

1
Maxwell Finland Laboratory for Infectious Diseases, Boston University School of Medicine and Boston Medical Center, Boston, MA, 02118, USA. khsu@bu.edu

Abstract

The diagnostic value of negative blood cultures from neonates whose mothers receive intrapartum antimicrobial prophylaxis for prevention of perinatal group B streptococcal disease is uncertain. We investigated whether blood culture medium containing resin designed to adsorb antibiotic improved group B streptococcal detection following simulated intrapartum antimicrobial prophylaxis. Group B streptococcus (Streptococcus agalactiae) was preincubated with varying antibiotic concentrations before inoculation into BACTEC Peds Plus resin-containing medium, BACTEC Standard, or Trek ESP 80A. In the presence of 10 mcg/mL ampicillin, detection of both low (<500 CFU/mL) and high (>500 CFU/mL) S. agalactiae inocula ranged between 75-100% of resin-containing medium bottles; detection rates in both non-resin-containing media were lower. When S. agalactiae was detected, it was detected sooner with resin-containing medium. The addition of gentamicin to ampicillin did not affect sensitivity of resin-containing medium for S. agalactiae. In our model, resin-containing medium more consistently and more rapidly detected S. agalactiae than did either of two non-resin-containing media, in the presence of antibiotic levels likely present in fetal sera following intrapartum antimicrobial prophylaxis.

PMID:
12573547
[Indexed for MEDLINE]

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