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Ann Clin Biochem. 2002 Nov;39(Pt 6):612-3.

An improved quantitative assay of glycogen in erythrocytes.

Author information

1
Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku-ku Nagoya 468-8503, Japan. miwaichi@ccmfs.meijo-u.ac.jp

Abstract

BACKGROUND:

Current methods for the quantitative determination of glycogen in erythrocytes are unsatisfactory.

METHODS:

Erythrocytes were deproteinized with perchloric acid either after haemolysing by freezing and thawing twice or without freezing and thawing, and the glycogen content was determined by an enzymatic method.

RESULTS:

Freezing and thawing resulted in a significantly higher glycogen content, and the recovery of added glycogen using this method was nearly 100%. The mean erythrocyte glycogen content in healthy volunteers (n = 17) was 69.5 microg/g haemoglobin, a value much higher than the previously reported values in healthy subjects.

CONCLUSION:

Freezing and thawing improves the assay of erythrocyte glycogen.

PMID:
12564847
DOI:
10.1177/000456320203900613
[Indexed for MEDLINE]

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