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J Craniomaxillofac Surg. 2003 Feb;31(1):27-33.

Bone regeneration following injection of mesenchymal stem cells and fibrin glue with a biodegradable scaffold.

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1
Department of Oral and Maxillofacial Surgery, Nagoya University, Graduate School of Medicine, Japan

Abstract

AIM:

The purpose of this study was to determine whether a combination of fibrin glue, beta-tricalcium phosphate as a biodegradable (beta-TCP) and mesenchymal stem cells would provide three-dimensional templates for bone growth resulting in new bone formation at heterotopic sites in the rat with plasticity.

MATERIAL AND METHODS:

Growing stem cells and developing matrices, explanted from the rat femur, were fragmented and mixed with fibrin glue in a syringe. The cells/beta-TCP fibrin glue admixtures were injected into the subcutaneous space on the dorsum of the rat.

RESULTS:

Eight weeks after implantation, gross morphology revealed a pearly opalescence and firm consistency. Histological inspections showed newly formed bone structures in all admixtures, but none in the control groups when only fibrin glue and beta-TCP were injected. Osteopontin, a protein important in bone development, was identified by using antibodies in all cells/beta-TCP fibrin glue admixtures.

CONCLUSION:

Mesenchymal stem cells/beta-TCP fibrin glue admixtures can result in successful bone formation. This technique holds the promise of a minimally invasive means of generating autogenous bone to correct or reconstruct bony defects.

PMID:
12553923
[Indexed for MEDLINE]
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