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J Oral Pathol Med. 2003 Feb;32(2):77-83.

Th1 cytokines in oral lichen planus.

Author information

1
Oral Biology and Pathology, The University of Queensland, St Lucia, Brisbane, Queensland, Australia.

Abstract

BACKGROUND:

Cell-mediated immune responses in oral lichen planus (OLP) may be regulated by cytokines and their receptors.

METHODS:

In situ cytokine expression and in vitro cytokine secretion in OLP were determined by immunohistochemistry and ELISA.

RESULTS:

The majority of subepithelial and intraepithelial mononuclear cells in OLP were CD8+. In some cases, intraepithelial CD8+ cells were adjacent to degenerating keratinocytes. CD4+ cells were observed mainly in the deep lamina propria with occasional CD4+ cells close to basal keratinocytes. Mononuclear cells expressed IFN-gamma in the superficial lamina propria and TNF-alpha adjacent to basal keratinocytes. Basal keratinocytes expressed TNF-alpha as a continuous band. TNF R1 was expressed by mononuclear cells and basal and suprabasal keratinocytes. There was variable expression of TGF-beta1 in the subepithelial infiltrate while all intraepithelial mononuclear cells were TGF-beta1-. Keratinocytes in OLP stained weakly for TGF-beta1. Unstimulated OLP lesional T cells secreted IFN-gamma in vitro. TNF-alpha stimulation down-regulated IFN-gamma secretion and up-regulated TNF-alpha secretion. IL-4, IL-10 and TGF-beta1 secretion were not detected.

CONCLUSIONS:

These data suggest the development of a T helper 1 immune response that may promote CD8+ cytotoxic T-cell activity in OLP.

[Indexed for MEDLINE]

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