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Nucleic Acids Res. 2003 Jan 15;31(2):E6-6.

Improving baculovirus recombination.

Author information

1
Animal and Microbial Sciences, University of Reading, Whiteknights, Reading RG6 6AJ, UK.

Abstract

Recombinant baculoviruses have established themselves as a favoured technology for the high-level expression of recombinant proteins. The construction of recombinant viruses, however, is a time consuming step that restricts consideration of the technology for high throughput developments. Here we use a targeted gene knockout technology to inactivate an essential viral gene that lies adjacent to the locus used for recombination. Viral DNA prepared from the knockout fails to initiate an infection unless rescued by recombination with a baculovirus transfer vector. Modified viral DNA allows 100% recombinant virus formation, obviates the need for further virus purification and offers an efficient means of mass parallel recombinant formation.

PMID:
12527795
PMCID:
PMC140531
DOI:
10.1093/nar/gng006
[Indexed for MEDLINE]
Free PMC Article

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