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Biochemistry. 2003 Jan 21;42(2):257-64.

LytG of Bacillus subtilis is a novel peptidoglycan hydrolase: the major active glucosaminidase.

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1
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, United Kingdom.

Abstract

LytG (YubE) of Bacillus subtilis is a novel 32 kDa autolysin produced during vegetative growth under the control of Esigma(A) RNA polymerase. Muropeptide analysis of vegetative cells of B. subtilis revealed LytG to be the major glucosaminidase responsible for peptidoglycan structural determination during vegetative growth. Overexpression and purification of LytG allowed its biochemical characterization. Despite sequence homology suggesting muramidase activity, LytG is a novel glucosaminidase with exoenzyme activity and may form part of a novel family of autolysins. It is involved in cell division, lysis, and motility on swarm plates.

PMID:
12525152
DOI:
10.1021/bi020498c
[Indexed for MEDLINE]
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