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Planta. 2003 Jan;216(3):502-11. Epub 2002 Aug 10.

In vitro biosynthesis of 1,4-beta-galactan attached to a pectin-xyloglucan complex in pea.

Author information

1
Department of Biology, American University of Beirut, Beirut, Lebanon.

Abstract

Particulate enzyme preparations were prepared from etiolated pea ( Pisum sativum L.) epicotyls and used to assay for 1,4-beta-galactan synthase using UDP-[U-(14)C]galactose. Optimum conditions for 1,4-beta-galactan synthesis were determined. The enzyme products were characterized by selective enzymic degradation, gel permeation chromatography and anion-exchange chromatography. Evidence was obtained for the formation of 1,4-beta-galactan chain attached to a pectic backbone containing both polygalacturonic acid and rhamnogalacturonan I. The results also indicated that part or all of this nascent pectin was present as a complex with xyloglucan.

PMID:
12520343
DOI:
10.1007/s00425-002-0861-y
[Indexed for MEDLINE]

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