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Biochem Biophys Res Commun. 2003 Jan 17;300(3):645-8.

Purification of antifreeze proteins by adsorption to ice.

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1
Department of Biology, Queen's University, Kingston, Ont., Canada K7L 3N6.

Abstract

Antifreeze proteins (AFPs) can protect organisms from freezing injury by adsorbing to ice and inhibiting its growth. We describe here a method where ice, grown on a cold finger, is used to selectively adsorb and purify these ice-binding proteins from a crude mixture. Type III recombinant AFP was enriched approximately 50-fold after one round of partitioning into ice and purified to homogeneity by a second round. This method can also be used to purify non-ice-binding proteins by linkage to AFP domains as demonstrated by the recovery of a 50 kDa maltose-binding protein-AFP fusion from a crude lysate of Escherichia coli.

PMID:
12507497
[Indexed for MEDLINE]

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