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Biochem Biophys Res Commun. 2003 Jan 17;300(3):645-8.

Purification of antifreeze proteins by adsorption to ice.

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  • 1Department of Biology, Queen's University, Kingston, Ont., Canada K7L 3N6.


Antifreeze proteins (AFPs) can protect organisms from freezing injury by adsorbing to ice and inhibiting its growth. We describe here a method where ice, grown on a cold finger, is used to selectively adsorb and purify these ice-binding proteins from a crude mixture. Type III recombinant AFP was enriched approximately 50-fold after one round of partitioning into ice and purified to homogeneity by a second round. This method can also be used to purify non-ice-binding proteins by linkage to AFP domains as demonstrated by the recovery of a 50 kDa maltose-binding protein-AFP fusion from a crude lysate of Escherichia coli.

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