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J Immunol Methods. 2003 Jan 15;272(1-2):177-87.

Identification of an antigen-specific B cell population.

Author information

1
Room 405, Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Forch. Building, Bronx, NY 10461, USA.

Abstract

The difficulty in characterizing antigen-specific B cells that arise in the native B cell repertoire has been a formidable obstacle to understanding both protective and pathogenic antibody responses. We have developed a tetramer-based technique for identifying antigen-specific B cells. Biotin-labeled antigen is made tetrameric by interaction with streptavidin. The enhanced avidity of this antigenic compound for the B cell membrane permits the visualization, characterization and isolation of antigen-specific B cells.

PMID:
12505722
DOI:
10.1016/s0022-1759(02)00499-4
[Indexed for MEDLINE]

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