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Cell Struct Funct. 2002 Oct;27(5):349-55.

Spinning-disk confocal microscopy -- a cutting-edge tool for imaging of membrane traffic.

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1
Molecular Membrane Biology Laboratory, RIKEN Discovery Research Institute, Saitama, Japan. nakano@postman.riken.go.jp

Abstract

Confocal laser scanning microscopy is experiencing a revolution in speed from the world of seconds to that of milliseconds. The spinning Nipkow disk method with microlenses has made this remarkable innovation possible. In combination with the ultrahigh-sensitivity, high-speed and high-resolution camera system based on avalanche multiplication of photoconduction, we are now able to observe the extremely dynamic movement of small vesicles in living cells in real time.

PMID:
12502889
[Indexed for MEDLINE]
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