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J Virol. 2003 Jan;77(2):1644-8.

Yellow fever virus replicons as an expression system for hepatitis C virus structural proteins.

Author information

1
Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, The Netherlands.

Abstract

Chimeric yellow fever virus (YF) RNAs were constructed in which the YF structural genes were replaced by the hepatitis C virus (HCV) structural genes or fusions between the YF and HCV structural genes. Interestingly, RNA replication required nucleotide complementarity between the 3'-located conserved sequence 1 and an RNA sequence located in the 5' end of the YF capsid sequence. The (chimeric-)HCV structural proteins were efficiently expressed and processed, and the native E1/E2 heterodimer was formed. However, no indication for the production of HCV-like particles was obtained.

PMID:
12502883
PMCID:
PMC140782
DOI:
10.1128/jvi.77.2.1644-1648.2003
[Indexed for MEDLINE]
Free PMC Article

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