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Phytochem Anal. 2002 Nov-Dec;13(6):349-53.

Application of centrifugal force to the extraction and separation of parasorboside and gerberin from Gerbera hybrida.

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Department of Pharmacy, Division of Pharmacognosy, University of Helsinki, PO Box 56, Helsinki FIN-00014, Finland.


A method for the separation of parasorboside and gerberin from the ornamental plant Gerbera hybrida (Asteraceae) has been developed. The two closely related glucosides were extracted using an Extrachrom instrument, a prototype multi-functional separation tool equipped with an extraction chamber. The rotation planar extraction procedure was compared with that of a medium pressure solid-liquid extraction system. The resulting extracts were pre-purified using rotation planar chromatography and the results compared with those obtained using medium pressure liquid chromatography with silica gel as the stationary phase and a mobile phase of methanol:ethyl acetate:tetrahydrofuran at selectivity point Ps = 111 with 1% formic acid as modifier. The title compounds were isolated from the purified extracts by TLC and their structures confirmed by 1H- and 13C-NMR spectroscopy.

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