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Phytochem Anal. 2002 Nov-Dec;13(6):343-8.

Analysis and quantitative determination of group B saponins in processed soybean products.

Author information

1
United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research Center, 1815 North University Street, Peoria, Illinois, USA. berhowma@ncaur.usda.gov

Abstract

Pure analytical standards for the major saponins present in processed soy products, the group B saponins (soyasaponins I, II, III and IV), were isolated in mg quantities by a combination of processing, precipitation/re-solubilisation, TLC and preparative HPLC. These standards were determined to be pure by LC-ESI/MS analysis and NMR. The standards were used to perfect a facile analytical HPLC method using spectrometric detection to determine the percent composition of the group B soyasaponins in various products from processing of soybean.

PMID:
12494753
DOI:
10.1002/pca.664
[Indexed for MEDLINE]

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