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Phytochem Anal. 2002 Nov-Dec;13(6):343-8.

Analysis and quantitative determination of group B saponins in processed soybean products.

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United States Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research Center, 1815 North University Street, Peoria, Illinois, USA.


Pure analytical standards for the major saponins present in processed soy products, the group B saponins (soyasaponins I, II, III and IV), were isolated in mg quantities by a combination of processing, precipitation/re-solubilisation, TLC and preparative HPLC. These standards were determined to be pure by LC-ESI/MS analysis and NMR. The standards were used to perfect a facile analytical HPLC method using spectrometric detection to determine the percent composition of the group B soyasaponins in various products from processing of soybean.

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