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Chin Med J (Engl). 2002 Oct;115(10):1573-5.

Study of isolation of fluoroquinolone-resistant Ureaplasma urealyticum and identification of mutant sites.

Author information

1
Department of Microbiology, Basic Medical College, Nanhua University, Hengyang 421001, Hunan Province, China.

Abstract

OBJECTIVE:

To study the resistance mechanism of clinical isolates of Ureaplasma urealyticum resistant to fluoroquinolones.

METHODS:

Thirteen isolates of Ureaplasma urealyticum resistant to six fluoroquinolones were selected out of 184 clinical isolates and their QRDRs (quinolone resistance-determining region) gyrA, gyrB, parC and parE were amplified by PCR. Sequencing results were compared to those susceptible reference strains and a comparison of deduced amino acid sequences were performed.

RESULTS:

Sequence comparison revealed a C to A change at 87nt of gyrA QRDR leading to the substitution of Asp95 with glutamic acid and a C to T change at 50nt of parC QRDR leading to the substitution of Ser80 with leucine.

CONCLUSION:

These results suggest that a C to A change at 87nt of gyrA QRDR and a C to T change at 50nt of parC QRDR are associated with fluoroquinolone resistance of Ureaplasma urealyticum.

PMID:
12490114
[Indexed for MEDLINE]

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