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Methods Cell Sci. 2001;23(4):205-9.

Preparation of dissociated zebrafish spinal neuron cultures.

Author information

1
University of California at Berkeley, Department of Molecular and Cellular Biology, 142 Life Sciences Addition #3200, Berkeley, CA 94720-3200, USA. soren@sorenandersen1.org

Abstract

The method described here explains a simple protocol for how to prepare dissociated Zebrafish spinal neuron cultures. The neurons grow fast in a simple culture medium and at room temperature. Considering the advantages afforded by the optical transparency of the Zebrafish embryo combined with the powerful molecular perturbation techniques available, this technique has potential to further advance molecular analysis of axon growth and guidance.

PMID:
12486331
[Indexed for MEDLINE]

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