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Trends Biotechnol. 2003 Jan;21(1):45-52.

Microbial cell-surface display.

Author information

1
Metabolic and Biomolecular Engineering National Research Laboratory, Daejeon, South Korea. leesy@mail.kaist.ac.kr

Abstract

Cell-surface display allows peptides and proteins to be displayed on the surface of microbial cells by fusing them with the anchoring motifs. The protein to be displayed - the passenger protein - can be fused to an anchoring motif - the carrier protein - by N-terminal fusion, C-terminal fusion or sandwich fusion. The characteristics of carrier protein, passenger protein and host cell, and fusion method all affect the efficiency of surface display of proteins. Microbial cell-surface display has many potential applications, including live vaccine development, peptide library screening, bioconversion using whole cell biocatalyst and bioadsorption.

PMID:
12480350
DOI:
10.1016/s0167-7799(02)00006-9
[Indexed for MEDLINE]

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