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Plant J. 2002 Dec;32(5):631-9.

Polyribosome loading of spinach mRNAs for photosystem I subunits is controlled by photosynthetic electron transport.

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Institut für Allgemeine Botanik, Lehrstuhl Pflanzenphysiologie, Friedrich-Schiller-Universität Jena, Dornburger Str. 159, Germany.


In light-, but not in dark-grown spinach seedlings, the mRNAs for the nuclear-encoded photosystem I subunits D, F and L are associated with polyribosomes and this association is prevented by the application of 3-(3',4'-dichlorophenyl)-1,1'-dimethyl urea (DCMU), an inhibitor of the photosynthetic electron transport. To identify the cis-elements which are responsible for this regulation, we generated a series of chimeric PsaD constructs and tested them in transgenic tobacco. The spinach PsaD 5'-untranslated region is sufficient to confer light- and photosynthesis-dependent polyribosome association onto the uidA reporter gene, while the tobacco PsaD 5'-untranslated region directs constitutive polyribosome association. These results are discussed with regard to signals from photosynthetic electron flow which control processes in the cytoplasm.

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