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Mol Genet Genomics. 2002 Dec;268(4):518-24. Epub 2002 Oct 29.

Transcriptional analysis of the pst operon of Escherichia coli.

Author information

1
Departamento de Microbiologia Instituto de Ciências Biomédicas Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374 São Paulo-SP CEP 05508-900, Brazil.

Abstract

The pst operon of Escherichia coli, which encodes the phosphate-specific transport system, is composed of five genes, pstS, pstC, pstA, pstB and phoU, whose transcription is induced by phosphate starvation. A phosphate-regulated promoter located upstream of the most proximal gene ( pstS) controls the transcription of the entire operon. Though the full-length pst mRNA could be detected by an improved RT-PCR protocol, Northern analysis using several pst-specific probes failed to reveal this transcript. Instead, smaller but distinct pst mRNA species were evident. Primer-extension experiments localized the 5' ends of pst mRNAs within the operon. The data suggest that the full-length mRNA is rapidly processed post-transcriptionally.

PMID:
12471449
DOI:
10.1007/s00438-002-0764-4
[Indexed for MEDLINE]

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