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J Biol Chem. 2003 Jan 31;278(5):3204-9. Epub 2002 Nov 22.

Cinnamycin (Ro 09-0198) promotes cell binding and toxicity by inducing transbilayer lipid movement.

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  • 1Supra-Biomolecular System Research Group, RIKEN Institute of Physical and Chemical Research, Frontier Research System, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.

Abstract

Cinnamycin is a unique toxin in that its receptor, phosphatidylethanolamine (PE), resides in the inner layer of the plasma membrane. Little is known about how the toxin recognizes PE and causes cytotoxicity. We showed that cinnamycin induced transbilayer phospholipid movement in target cells that leads to the exposure of inner leaflet PE to the toxin. Model membrane studies revealed that cinnamycin induced transbilayer lipid movement in a PE concentration-dependent manner. Re-orientation of phospholipids was accompanied by an increase in the incidence of beta-sheet structure in cinnamycin. When the surface concentration of PE was high, cinnamycin induced membrane re-organization such as membrane fusion and the alteration of membrane gross morphology. These results suggest that cinnamycin promotes its own binding to the cell and causes toxicity by inducing transbilayer lipid movement.

PMID:
12446685
DOI:
10.1074/jbc.M210347200
[PubMed - indexed for MEDLINE]
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