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Trends Genet. 2002 Dec;18(12):613-5.

Web-based primer design for single nucleotide polymorphism analysis.

Author information

1
Dept of Biology, Washington University, Campus Box 1137, St Louis, MO 63130-4899, USA. mneff@biology2.wustl.edu

Abstract

The detection of single nucleotide polymorphisms by PCR is necessary for many types of genetic analysis, from mapping genomes to tracking specific mutations. This technique is most commonly used when polymorphisms alter restriction endonuclease recognition sites. Here we describe a web-based program, dCAPS Finder 2.0, that facilitates the design of mismatched PCR primers to create or remove a restriction endonuclease recognition site relative to the polymorphism being analyzed.

PMID:
12446140
DOI:
10.1016/s0168-9525(02)02820-2
[Indexed for MEDLINE]

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