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J Am Chem Soc. 2002 Nov 27;124(47):13994-5.

D/H amide isotope effect in model alpha-helical peptides.

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Department of Chemistry, New York University, 100 Washington Place, New York, NY 10003, USA.


The contribution of amide related hydrogen bonds to protein stability has recently been evaluated using the "Cm experiment", which measures the D/H amide isotope effect in proteins. We show here using isolated alpha-helical peptides that there is a significant effect of denaturant concentration on the measured D/H isotope effect, and that valid comparison of different proteins requires correcting for differences in denaturant (GdmHCl) concentration. Finally our results suggest that H-bonds in an isolated alpha-helix may contribute more to helix stability because of less strain compared to those in helical proteins and that the buried helical H-bonds in helical proteins are not necessarily energetically more favorable than solvent exposed H-bonds in isolated helices.

[Indexed for MEDLINE]

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