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Infect Immun. 2002 Dec;70(12):7120-5.

Construction of an Actinobacillus pleuropneumoniae serotype 2 prototype live negative-marker vaccine.

Author information

1
Institut für Mikrobiologie und Tierseuchen, Tieraerztliche Hochschule Hannover, Germany.

Abstract

Deletions were introduced into the ureC and apxIIA genes of an Actinobacillus pleuropneumoniae serotype 2 strain by homologous recombination and counterselection. The double-mutant contains no foreign DNA, is highly attenuated, protects pigs from homologous challenge upon a single aerosol application, and facilitates the serological discrimination of immunized and infected herds.

PMID:
12438394
PMCID:
PMC133062
DOI:
10.1128/iai.70.12.7120-7125.2002
[Indexed for MEDLINE]
Free PMC Article

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