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Methods. 2002 Nov;28(3):323-35.

Group II introns: highly specific endonucleases with modular structures and diverse catalytic functions.

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1
Department of Biochemistry and Molecular Biophysics, 630 West 168 Street, Box 36, Columbia University, New York, NY 10032, USA.

Abstract

Group II introns are large catalytic RNAs with a remarkable repertoire of reactions. Here we present construct designs and protocols that were used to develop a set of kinetic frameworks for studying the structure and reaction mechanisms of group II introns and ribozymes derived from them. In addition, we discuss application of these systems to structure/function analysis of the ai5gamma group II intron.

PMID:
12431436
[Indexed for MEDLINE]

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