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Arthritis Rheum. 2002 Nov;46(11):2972-82.

Cell-surface expression and immune receptor recognition of HLA-B27 homodimers.

Author information

1
Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.

Abstract

OBJECTIVE:

HLA-B27 is capable of forming in vitro a heavy-chain homodimer structure lacking beta(2)-microglobulin. We undertook this study to ascertain if patients with spondylarthritis express beta(2)-microglobulin-free HLA-B27 heavy chains in the form of homodimers and receptors for HLA-B27 homodimers.

METHODS:

Expression of HLA-B27 heavy chains by mononuclear cells was analyzed by fluorescence-activated cell sorter staining, Western blotting with the monoclonal antibody HC-10, and 2-dimensional isoelectric focusing. Fluorescence-labeled tetrameric complexes of HLA-B27 heavy-chain homodimers were constructed in which each dimer comprised one His-tagged heavy chain and one biotinylated heavy chain, and were used to stain patient and control mononuclear cells and transfected cell lines.

RESULTS:

Patients with spondylarthritis expressed cell-surface HLA-B27 homodimers. Populations of synovial and peripheral blood monocytes, and B and T lymphocytes from patients with spondylarthritis, and controls carried receptors for HLA-B27 homodimers. Experiments with transfected cell lines demonstrated that KIR3DL1 and KIR3DL2, and immunoglobulin-like transcript 4 (ILT4), but not ILT2, are receptors for HLA-B27 homodimers.

CONCLUSION:

Patients with spondylarthritis express both HLA-B27 heavy-chain homodimers and receptors for HLA-B27 homodimers. This may be of significance with regard to disease pathogenesis.

PMID:
12428240
DOI:
10.1002/art.10605
[Indexed for MEDLINE]
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