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J Neurosci Methods. 2002 Nov 15;121(1):41-52.

Kinetic analysis of striatal clearance of exogenous dopamine recorded by chronoamperometry in freely-moving rats.

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1
Department of Pharmacology, Neuroscience Program, University of Colorado Health Sciences Center, Box C-236, 4200 East Ninth Avenue, Denver, CO 80262, USA. jilla.sabeti@uchsc.edu

Abstract

Previously, we developed technology that coupled high-speed chronoamperometry with microejections of dopamine (DA) to measure DA clearance in the brains of freely-behaving rats. Here, by varying the ejection volumes of DA across a 200-fold difference, the kinetics of striatal clearance were analyzed as a function of time and DA volume from 289 chronoamperometric signals (n=20 rats). Each DA clearance trace was fitted to a first-order exponential decay function to determine the rate constant for DA clearance (k). Additionally, the apparent Michaelis-Menten V(max) and K(m) kinetic constants were determined in freely-moving rats, enabling quantitative comparison of our values with other models of reuptake. The first-order rate constant for DA clearance, which reflects the V(max)/K(m) ratio or clearance efficiency, did not vary significantly when small volumes of DA were ejected resulting in peak DA signal amplitudes (A(max)) of <5 microM. However, following nomifensine-induced DAT inhibition, A(max) was increased and k was attenuated simultaneously with behavioral activation; and A(max) and behavior remained elevated beyond the initial period. Our results indicate that the analysis of kinetic parameters from chronoamperometric DA signals may be useful for investigating drug-induced regulation of DAT kinetics in relation to the behavior of freely-moving rats.

PMID:
12393160
DOI:
10.1016/s0165-0270(02)00229-7
[Indexed for MEDLINE]

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