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J Mol Cell Cardiol. 2002 Oct;34(10):1309-16.

Regulation of cardiac fibroblast cellular function by leukemia inhibitory factor.

Author information

1
Winters Center for Heart Failure Research, Baylor College of Medicine, 6565 Fannin Street, Houston, TX 77030, USA.

Abstract

BACKGROUND:

Previous studies have shown that leukemia inhibitory factor (LIF) provokes hypertrophic and cytoprotective effects in cardiac myocytes. However, the effects of LIF in cardiac fibroblasts are not known. Given that the cardiac fibroblast is the most abundant cell type in the heart, we sought to examine the functional effects of LIF on cardiac fibroblasts in vitro.

RESULTS:

Short-term LIF stimulation (24h) had no effect on fibroblast proliferation and/or cell differentiation. However, longer-term LIF stimulation (48-72h) increased fibroblast proliferation, and significantly inhibited cardiac fibroblast differentiation into myofibroblasts. Moreover, 72h of LIF stimulation significantly reduced collagen content in cardiac fibroblasts cultures, as well as decreased MMP activity in fibroblast culture supernatants.

CONCLUSION:

The results of this study suggest that LIF stimulation down-regulates several key components of the remodeling process, including collagen content and matrix metalloproteinase (MMP) activation, and thus suggest that LIF may play an important autocrine/paracrine role in preventing excessive extracellular matrix remodeling following acute myocardial injury.

PMID:
12392991
DOI:
10.1006/jmcc.2002.2059
[Indexed for MEDLINE]

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