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J Appl Microbiol. 2002;93(5):758-64.

Identification of the O-antigen biosynthesis genes of Escherichia coli O91 and development of a O91 PCR serotyping test.

Author information

1
Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches sur l'Hygiène et la Qualité des Aliments, Unité: Atelier de Biotechnologie, Maisons-Alfort, France. s.perelle@afssa.fr

Abstract

AIMS:

The aims of the study were to characterize the O91 O-antigen gene cluster from Shiga toxin-producing Escherichia coli (STEC) O91 and to provide the basis for a specific PCR test for rapid detection of E. coli O91.

METHODS AND RESULTS:

The published primers complementary to JUMPstart and gnd gene, the conserved flanking sequences of O-antigen genes clusters in E. coli and related species were used to amplify the 10-kbp O91 O-antigen biosynthesis locus of STEC O91. A DNA library representative of this cluster allowed two O91 specific probes to be identified, and two specific PCR O91 serotyping tests to be successfully developed.

CONCLUSIONS:

These results confirm that the O-antigen gene cluster sequences of E. coli allow rapidly a specific O-antigen PCR assay to be designed.

SIGNIFICANCE AND IMPACT OF THE STUDY:

These findings increase the number of PCR-assays available to replace the classical O-serotyping among E. coli O-antigen.

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