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J Microsc. 2002 Oct;208(Pt 1):3-10.

Freeze substitution of high-pressure frozen samples: the visibility of biological membranes is improved when the substitution medium contains water.

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1
Zentrale Einrichtung Elektronenmikroskopie, Universitaet Ulm, Albert-Einstein-Allee 11, 89069 Ulm, Germany. paul.walther@medzin.uni-ulm.de

Abstract

Biological membranes are often poorly visible with the electron microscope after high-pressure freezing and freeze-substitution. The water content of the sample and of the substitution medium is one factor among others that strongly influences membrane visibility. In order to investigate this effect, high-pressure frozen yeast cells, rat-pancreas tissue and arthropod tissue were freeze-substituted with and without adding water to the substitution medium. The visibility of the biological membranes was generally improved if the substitution medium contained 1-5% water. The effect was especially pronounced in yeast cells, where membrane visibility was poor after freeze-substitution with water-free medium but good after addition of 5% water to the substitution medium.

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