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Gastroenterology. 2002 Oct;123(4):1135-43.

Neurotensin receptor-1 and -3 complex modulates the cellular signaling of neurotensin in the HT29 cell line.

Author information

1
Institut de Pharmacologie Mol├ęculaire et Cellulaire, Sophia Antipolis, Valbonne, France.

Abstract

BACKGROUND & AIMS:

The neuropeptide neurotensin (NT) exerts its intracellular effect by interacting with 3 different receptors. Two of these receptors (NTR1 and NTR2) belong to the G protein-coupled receptor family, whereas the third one (NTR3) is a type I receptor with a single transmembrane domain. We recently showed that the 2 structurally different receptors NTR1 and NTR3 were coexpressed in several human cancer cells on which NT exerts proliferative effects.

METHODS:

Here, by an immunoprecipitation approach, we provide biochemical evidence for an endogenous heterodimerization of the G protein-coupled receptor NTR1 with the NTR3 in the human adenocarcinoma cell line HT29.

RESULTS:

We show that both receptors are expressed and colocalized within the cell surface of HT29 cells where they already interact to form a heterodimer. The NTR1-NTR3 complex is then internalized on NT stimulation.

CONCLUSIONS:

The complex formed between these 2 structurally unrelated NT receptors modulates both the NT-induced phosphorylation of mitogen-activated protein kinases and the phosphoinositide (PI) turnover mediated by the NTR1.

PMID:
12360476
DOI:
10.1053/gast.2002.36000
[Indexed for MEDLINE]

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