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Lett Appl Microbiol. 2002;35(4):267-71.

Preparation of microparticulate beta-glucan from Saccharomyces cerevisiae for use in immune potentiation.

Author information

1
Department of Microbiology, University of Nevada School of Medicine, Reno, NV 89557, USA. khunter@unr.edu

Abstract

AIMS:

To develop a method for the preparation of an immunologically active, homogeneous, nonaggregated, microparticulate beta-glucan-containing material from the budding yeast Saccharomyces cerevisiae.

METHODS AND RESULTS:

Using a combination of sonication and spray-drying, a homogeneous preparation of 1-2-mu diameter beta-glucan-containing particles was made from alkali- and acid-insoluble yeast cell wall material. This microparticulate beta-glucan remained in suspension longer and, following oral administration at 0.1 mg kg(-1) for 14 d, enhanced phagocytosis of mouse peritoneal macrophages significantly better than did aggregated beta-glucan particles.

CONCLUSIONS:

A new sonication and spray-drying method can be employed to overcome the problem of aggregation of beta-glucan microparticles in aqueous media.

SIGNIFICANCE AND IMPACT OF THE STUDY:

A microparticulate form of beta-glucan that remains in suspension longer for pharmaceutical applications and has superior immune potentiation characteristics has been developed.

PMID:
12358685
[Indexed for MEDLINE]
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