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Lasers Surg Med. 2002;31(4):263-7.

Effect of low-power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts.

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1
Department of Stomatology, School of Dentistry, University of São Paulo-SP, Brazil 05508-900.

Abstract

BACKGROUND AND OBJECTIVES:

In dentistry, low-power lasers have been used in the treatment of dentin hypersensitivity, gingivitis, periodontitis, and different forms of oral ulcers. This in vitro study focuses on the biostimulation of NIH-3T3 fibroblasts by a low-power Ga-As-pulsed laser.

STUDY DESIGN/MATERIALS AND METHODS:

We have studied cell growth and procollagen synthesis of cultured fibroblasts submitted to low-power laser irradiation with energy densities varying from 3 to 5 J/cm(2) over a period of 1-6 days. The light source was a 120 mW Ga-As diode laser (lambda = 904 nm). Growth curves and procollagen immunoprecipitation were obtained.

RESULTS:

Irradiation of 3 and 4 J/cm(2) increased the cell numbers about threefold to sixfold comparing to control cultures. However, this effect was restricted to a small range of energy densities since 5 J/cm(2) had no effect on cell growth. The energy density of 3 J/cm(2) remarkably increased cell growth, with no effect on procollagen synthesis, as demonstrated by the immunoprecipitation analysis.

CONCLUSIONS:

Our results showed that a particular laser irradiation stimulates fibroblast proliferation, without impairing procollagen synthesis.

PMID:
12355572
DOI:
10.1002/lsm.10107
[Indexed for MEDLINE]
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