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Chromosoma. 2002 Sep;111(3):165-9. Epub 2002 May 30.

Simultaneous analysis of chromosomes and chromosome-associated proteins in mammalian oocytes and embryos.

Author information

1
Department of Genetics, Case Western Reserve University, Cleveland, Ohio, USA.

Abstract

Cytogenetic analyses of mammalian eggs and preimplantation embryos have been limited by the difficult and tedious task of preparing chromosomes from single cells or small numbers of cells. In this report we describe a new technique that is both reliable and comparatively simple. Further, since the technique does not use the conventional 3:1 methanol:acetic acid fixative, it has the advantage of producing high-resolution chromosome preparations without destroying chromosome-associated proteins. Thus, this method provides a sensitive means of conducting studies of a heretofore inaccessible period of mammalian development, and of studying proteins thought to mediate both meiotic chromosome segregation and chromatin modifications in the preimplantation embryo.

PMID:
12355205
DOI:
10.1007/s00412-002-0195-3
[Indexed for MEDLINE]

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