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Neuron. 2002 Sep 12;35(6):1099-110.

Altered presynaptic vesicle release and cycling during mGluR-dependent LTD.

Author information

1
Center for Neurobiology and Behavior, Department of Pharmacology, Howard Hughes Medical Institute, Columbia University, New York, NY 10032, USA.

Abstract

The site of modification of synaptic transmission during long-term plasticity in the mammalian hippocampus remains controversial. Here we used a fluorescent marker of presynaptic activity, FM 1-43, to directly image presynaptic function during metabotropic glutamate receptor-dependent long-term depression (mGluR-LTD) at CA3-CA1 excitatory synapses in acute hippocampal slices. We found a significant decrease in the rate of FM 1-43 release in response to synaptic stimulation following induction of mGluR-LTD, providing direct evidence for altered presynaptic function. Moreover, we found that mGluR-LTD causes several changes in FM dye release properties that are consistent with a change in the mode of vesicle cycling, possibly involving a switch from a full fusion mode of release to a "kiss-and-run" mode of release through the transient opening of a fusion pore.

PMID:
12354399
DOI:
10.1016/s0896-6273(02)00898-x
[Indexed for MEDLINE]
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