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Acta Crystallogr D Biol Crystallogr. 2002 Oct;58(Pt 10 Pt 2):1820-5. Epub 2002 Sep 28.

On the fitting of model electron densities into EM reconstructions: a reciprocal-space formulation.

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  • 1LVMS, CNRS-GIF, 91198 Gif-sur-Yvette, France.


A fast method for fitting model electron densities into EM reconstructions is presented. The methodology was inspired by the molecular-replacement technique, adapted to take into account phase information and the symmetry imposed during the EM reconstruction. Calculations are performed in reciprocal space, which enables the selection of large volumes of the EM maps, thus avoiding the bias introduced when defining the boundaries of the target density.

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