Effect of anoxia/reperfusion on the reversible active/de-active transition of NADH-ubiquinone oxidoreductase (complex I) in rat heart

Biochim Biophys Acta. 2002 Oct 3;1556(1):6-12. doi: 10.1016/s0005-2728(02)00280-3.

Abstract

The multi-subunit mammalian NADH-ubiquinone oxidoreductase (complex I) is part of the mitochondrial electron transport chain and physiologically serves to reduce ubiquinone with NADH as the electron donor. The three-dimensional structure of this enzyme complex remains to be elucidated and also little is known about the physiological regulation of complex I. The enzyme complex in vitro is known to exist as a mixture of active (A) and de-active (D) forms [Biochim. Biophys. Acta 1364 (1998) 169]. Studies are reported here examining the effect of anoxia and reperfusion on the A/D-equilibrium of complex I in rat hearts ex vivo. Complex I from the freshly isolated rat heart or after prolonged (1 h) normoxic perfusion exists in almost fully active form (87+/-2%). Either 30 min of nitrogen perfusion or global ischemia decreases the portion of active form of complex I to 40+/-2%. Upon re-oxygenation of cardiac tissue, complex I is converted back predominantly to the active form (80-85%). Abrupt alternation of anoxic and normoxic perfusion allows cycling between the two states of the enzyme. The possible role in the physiological regulation of complex I activity is discussed.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Electron Transport Complex I
  • Enzyme Activation
  • Ethylmaleimide
  • Heart / physiopathology*
  • Hypoxia / physiopathology*
  • In Vitro Techniques
  • Magnesium Chloride
  • Mitochondria, Heart / drug effects
  • Mitochondria, Heart / enzymology
  • Myocardial Reperfusion
  • Myocardium / enzymology
  • NADH, NADPH Oxidoreductases / chemistry
  • NADH, NADPH Oxidoreductases / metabolism*
  • Perfusion
  • Rats

Substances

  • Magnesium Chloride
  • NADH, NADPH Oxidoreductases
  • Electron Transport Complex I
  • Ethylmaleimide