DNA sequence analysis of DNA gyrase and DNA topoisomerase IV quinolone resistance-determining regions of Salmonella enterica serovar Typhi and serovar Paratyphi A

Antimicrob Agents Chemother. 2002 Oct;46(10):3249-52. doi: 10.1128/AAC.46.10.3249-3252.2002.

Abstract

The mutations that are responsible for fluoroquinolone resistance in the gyrA, gyrB, parC, and parE genes of Salmonella enterica serovar Typhi and serovar Paratyphi A were investigated. The sequences of the quinolone resistance-determining region of the gyrA gene in clinical isolates which showed decreased susceptibilities to fluoroquinolones had a single mutation at either the Ser-83 or the Asp-87 codon, and no mutations were found in the gyrB, parC, and parE genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Infective Agents / pharmacology*
  • DNA Gyrase / genetics*
  • DNA Topoisomerase IV / genetics*
  • Drug Resistance, Bacterial / genetics*
  • Fluoroquinolones
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Mutation
  • Salmonella paratyphi A / drug effects*
  • Salmonella paratyphi A / enzymology
  • Salmonella paratyphi A / genetics
  • Salmonella typhi / drug effects*
  • Salmonella typhi / enzymology
  • Salmonella typhi / genetics
  • Sequence Analysis, DNA

Substances

  • Anti-Infective Agents
  • Fluoroquinolones
  • DNA Topoisomerase IV
  • DNA Gyrase

Associated data

  • GENBANK/AB071870
  • GENBANK/AB071871
  • GENBANK/AB071987
  • GENBANK/AB072393
  • GENBANK/AB072396
  • GENBANK/AB072700
  • GENBANK/AB072701
  • GENBANK/AB072702