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Plant Physiol. 1994 Nov;106(3):1131-1136.

The H+/Cl- Symporter in Root-Hair Cells of Sinapis alba (An Electrophysiological Study Using Ion-Selective Microelectrodes).

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Botanisches Institut I, Universitat Giessen, Senckenbergstrasse 17, D-35390 Giessen, Germany.


In root-hair cells of Sinapis alba, cytosolic pH, cytosolic [Cl-], membrane potential, and membrane resistance have been measured to investigate proton-driven Cl- transport across the plasma membrane. Rapid lowering of the external pH transiently increased cytosolic [Cl-] and acidified the cytoplasm. To an abrupt increase in external [Cl-] the cells reacted with a rapid initial depolarization and a subsequent slower hyperpolarization, which was accompanied by an increase in cytosolic [Cl-] and [H+]. These results are indicative of an nH+/Cl- symport with n > 1. Simultaneous recording of the membrane potential, the proton motive force, cytosolic pH, and cytosolic [Cl-] reveals that kinetically this Cl- transport depends on the pH gradient across the plasma membrane rather than on the membrane potential.

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