Enzymatic production of cyclodextrins

Appl Microbiol Biotechnol. 2002 Sep;59(6):609-17. doi: 10.1007/s00253-002-1057-x. Epub 2002 Jul 16.

Abstract

Cyclodextrins (CD) are enzymatically modified starches with a wide range of applications in food, pharmaceutical and chemical industries, agriculture and environmental engineering. They are produced from starch via enzymatic conversion using cyclodextrin glycosyl transferases (CGTases) and partly alpha-amylases. Due to its low solubility in water, separation and purification of beta-CD is relatively easy compared to alpha- and gamma-CD. In recent years more economic processes for gamma-CD and especially alpha-CD production have been developed using improved CGTases and downstream processing. New purification steps, e.g. affinity adsorption, may reduce the use of complexing agents. The implementation of thermostable CGTases can simplify the production process and increase the selectivity of the reaction. A tabular overview of alpha-CD production processes is presented.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Bacillus / enzymology
  • Carbohydrate Sequence
  • Cyclodextrins / biosynthesis*
  • Cyclodextrins / metabolism
  • Glucosyltransferases / metabolism*
  • Molecular Sequence Data
  • Starch / metabolism
  • alpha-Amylases / metabolism*

Substances

  • Cyclodextrins
  • Starch
  • Glucosyltransferases
  • cyclomaltodextrin glucanotransferase
  • alpha-Amylases