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BMC Cell Biol. 2002 Sep 11;3:24.

Mutational analyses of the signals involved in the subcellular location of DSCR1.

Author information

1
Department of Genetics and Evolution, Federal University of São Carlos, Rodovia Washington Luiz km 235, São Carlos 13565-905, SP, Brazil. scpfister@uol.com.br

Abstract

BACKGROUND:

Down syndrome is the most frequent genetic disorder in humans. Rare cases involving partial trisomy of chromosome 21 allowed a small chromosomal region common to all carriers, called Down Syndrome Critical Region (DSCR), to be determined. The DSCR1 gene was identified in this region and is expressed preferentially in the brain, heart and skeletal muscle. Recent studies have shown that DSCR1 belongs to a family of proteins that binds and inhibits calcineurin, a serine-threonine phosphatase. The work reported on herein consisted of a study of the subcellular location of DSCR1 and DSCR1-mutated forms by fusion with a green fluorescent protein, using various cell lines, including human.

RESULTS:

The protein's location was preferentially nuclear, independently of the isoform, cell line and insertion in the GFP's N- or C-terminal. A segment in the C-terminal, which is important in the location of the protein, was identified by deletion. On the other hand, site-directed mutational analyses have indicated the involvement of some serine and threonine residues in this event.

CONCLUSION:

In this paper, we discuss the identification of amino acids which can be important for subcellular location of DSCR1. The involvement of residues that are prone to phosphorylation suggests that the location and function of DSCR1 may be regulated by kinases and/or phosphatases.

PMID:
12225619
PMCID:
PMC128833
[Indexed for MEDLINE]
Free PMC Article
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